FIG 5.

Knockdown with an independent siRNA confirmed that Sld5 depletion leads to multiple mitotic aberrations. (A) Control GL2 or SLD5 (2) (targeting a different region of Sld5) siRNA-transfected HeLa cells were costained for α-tubulin (green) and γ-tubulin (red) and for DNA with DAPI (blue). Control GL2 siRNA-transfected cells in different mitotic phases are shown. Examples of Sld5-depleted cells display an asymmetric mitotic spindle (red arrowhead in row iv), chromosome congression failure (yellow arrowhead in row iii), and decreased γ-tubulin staining at centrosomes (blue arrowhead in row v). (B) Quantification of spindle pole defects due to Sld5 depletion by siRNAs targeting different regions of Sld5 (expressed as percentages of total mitotic cells). The data are represented as the means and SD of the results of two independent experiments, with more than 40 cells analyzed in each sample. The P values were calculated using a two-tailed t test and show that the SLD5 siRNA-transfected samples were significantly different from control GL2 siRNA-transfected samples. *, P < 0.05. Scale bar, 10 μm.