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. 2017 Dec 1;12(1):33–47. doi: 10.1002/1878-0261.12146

Figure 4.

Figure 4

Combination of RO‐BIR2 and TRAIL enhances cell death of AML cells. FACS analysis of apoptotic population in U‐937 (A) or OCI‐AML3 (B) cells treated with either RO‐BIR, TRAIL single agents, or combination (n = 2, mean ± SD). Luminescent assays for caspase 3 and caspase 7 activities in OCI‐AML3 cells (C) or primary AML cells from sample SE211 (D) incubated with either RO‐BIR, TRAIL single agents, or combination at 24 h (n = 3, mean ± SD). (E) Western blot analysis for XIAP, caspase 3, cleaved (C) caspase 3, caspase 7, C‐caspase 7, PARP, and C‐PARP in OCI‐AML3 cells with different regimes as indicated. Lane 1, DMSO control; lane 2, RO‐BIR2 2 μm; lane 3, TRAIL 20 ng·mL−1; lane 4, TRAIL 10 ng·mL−1; lane 5, RO‐BIR2 2 μm+TRAIL 20 ng·mL−1; lane 6, RO‐BIR2 2 μm+ TRAIL 10 ng·mL−1. Beta‐actin served as loading control. Protein levels were determined by densitometric analysis. The experiments were duplicated and representative images were shown. (F) FACS analysis of apoptotic population in primary AML cells from SE112 case treated with either RO‐BIR, TRAIL single agents, or combinations (n = 2, mean ± SD).