TABLE 1.
Fatty acid compositions (% w/w) of the total lipid contents of yeast transformants harboring the control plasmid (pYES2) and the recombinant plasmids (pYES2-GcFADS2 and pYES2-TpFADS2) by adding 0.5 mM cis-LA or 0.5 mM cis-ALA or both substrates (0.25 mM cis-LA and 0.25 mM cis-ALA substrates)
| Transformants | |||||||||
| pYES2 (control) | pYES2-GcFADS2 | pYES2-TpFADS2 | |||||||
| Fatty acid | +LAa | +ALAa | +LA and +ALA | +LA | +ALA | +LA and +ALA | +LA | +ALA | +LA and +ALA |
| 16:0 (PA) | 21.9 ± 0.5 | 22.6 ± 0.3 | 23.6 ± 0.7 | 25.6 ± 0.1 | 23.5 ± 0.1 | 22.2 ± 0.5 | 22.2 ± 0.2 | 23.7 ± 0.5 | 25.5 ± 0.6 |
| 16:1 (PA) | 10.6 ± 0.2 | 11.7 ± 0.6 | 12.6 ± 0.3 | 11.3 ± 0.6 | 12.7 ± 0.3 | 11.1 ± 0.3 | 11.5 ± 0.1 | 13.3 ± 0.3 | 12.6 ± 0.5 |
| 18:0 (SA) | 10.2 ± 0.5 | 8.8 ± 0.5 | 9.3 ± 0.2 | 9.5 ± 0.3 | 9.6 ± 0.5 | 10.5 ± 0.6 | 9.3 ± 0.3 | 10.5 ± 0.5 | 9.3 ± 0.3 |
| 18:1 (OA) | 11.3 ± 0.5 | 12.5 ± 0.3 | 11.6 ± 0.2 | 13.1 ± 0.2 | 9.9 ± 0.2 | 12.2 ± 0.5 | 9.6 ± 0.6 | 10.2 ± 0.6 | 11.1 ± 0.5 |
| 18:2 (LA, ω-6) | 40.3 ± 0.3 | ND | 22.1 ± 0.5 | 38.0 ± 0.2 | ND | 18.1 ± 0.4 | 17.6 ± 0.3 | ND | 7.2 ± 0.4 |
| 18:3 (ALA, ω-3) | ND | 39.5 ± 0.2 | 21.5 ± 0.8 | ND | 37.4 ± 1.0 | 20.1 ± 0.3 | ND | 15.2 ± 0.6 | 5.0 ± 0.6 |
| 18:3 (GLA, ω-6) | ND | ND | ND | 1.9 ± 0.1 | ND | 1.1 ± 0.3 | 22.2 ± 0.5 | ND | 13.0 ± 0.5 |
| 18:4 (SDA, ω-3) | ND | ND | ND | ND | 2.8 ± 0.4 | 1.6 ± 0.1 | ND | 24.9 ± 0.9 | 15.5 ± 0.8 |
| LA conversion rateb | — | — | — | 4.9 ± 0.1 | — | 5.6 ± 0.8 | 55.6 ± 1.4 | — | 65.6 ± 1.8 |
| ALA conversion rateb | — | — | — | — | 7.1 ± 0.5 | 7.6 ± 0.6 | — | 62.3 ± 0.7 | 77.6 ± 1.9 |
a
There was no significantly difference in LA and ALA conversion rate between single- and double- substrate supplementation.
b
Conversion rate = 100 × ([product] / [product + substrate]). ND, not detected; —, none.