TABLE 2.

Fatty acid generation (mM) by yeast transformants expressing the wild-type and the mutant desaturases from TpFADS2

	Mutants
Fatty acid	TpFADS2	V295T	T299S	L303F	I306L	A315S	H360D	F368Y	S377M/L378M	H381N	L383I
0.25 mM cis-LA/ALA:
18:2 (LA)	0.081 ± 0.005	0.212 ± 0.002	0.124 ± 0.002	0.110 ± 0.001	0.170 ± 0.002	0.219 ± 0.001	0.149 ± 0.001	0.219 ± 0.002	0.199 ± 0.000	0.090 ± 0.002	0.100 ± 0.004
18:3 (ALA)	0.050 ± 0.005	0.219 ± 0.001	0.099 ± 0.000	0.119 ± 0.003	0.110 ± 0.009	0.228 ± 0.000	0.119 ± 0.001	0.230 ± 0.003	0.188 ± 0.002	0.089 ± 0.006	0.119 ± 0.005
18:3 (GLA)	0.164 ± 0.002	0.034 ± 0.002	0.130 ± 0.001	0.134 ± 0.002	0.077 ± 0.001	0.040 ± 0.001	0.111 ± 0.002	0.039 ± 0.000	0.057 ± 0.002	0.151 ± 0.004	0.141 ± 0.002
18:4 (SDA)	0.194 ± 0.001	0.038 ± 0.000	0.159 ± 0.003	0.136 ± 0.009	0.132 ± 0.000	0.028 ± 0.001	0.138 ± 0.003	0.013 ± 0.002	0.066 ± 0.006	0.166 ± 0.005	0.139 ± 0.000
LA %a	65.6 ± 1.8	13.6 ± 0.6	51.9 ± 0.9	53.6 ± 0.5	30.9 ± 0.9	15.9 ± 0.5	44.2 ± 0.3	15.6 ± 0.7	22.6 ± 0.1	60.5 ± 0.6	56.5 ± 1.5
ALA %a	77.6 ± 1.9	15.2 ± 0.3	63.6 ± 0.1	54.3 ± 1.1	52.6 ± 3.5	11.2 ± 0.1	55.3 ± 0.2	5.3 ± 1.3	26.3 ± 0.7	66.5 ± 2.2	55.5 ± 2.0
0.5 mM cis-LA/ALA:
18:2 (LA)	0.299 ± 0.009	0.449 ± 0.001	0.290 ± 0.000	0.317 ± 0.002	0.377 ± 0.000	0.439 ± 0.002	0.340 ± 0.002	0.420 ± 0.001	0.384 ± 0.000	0.286 ± 0.000	0.290 ± 0.005
18:3 (ALA)	0.249 ± 0.008	0.425 ± 0.000	0.240 ± 0.002	0.290 ± 0.002	0.299 ± 0.017	0.459 ± 0.004	0.286 ± 0.004	0.465 ± 0.006	0.389 ± 0.001	0.240 ± 0.010	0.285 ± 0.007
18:3 (GLA)	0.209 ± 0.001	0.060 ± 0.000	0.202 ± 0.002	0.193 ± 0.000	0.113 ± 0.002	0.070 ± 0.002	0.152 ± 0.001	0.072 ± 0.000	0.106 ± 0.000	0.204 ± 0.005	0.204 ± 0.000
18:4 (SDA)	0.259 ± 0.000	0.065 ± 0.002	0.251 ± 0.002	0.203 ± 0.017	0.210 ± 0.004	0.049 ± 0.004	0.204 ± 0.006	0.025 ± 0.001	0.120 ± 0.010	0.251 ± 0.007	0.205 ± 0.000
LA %a	41.8 ± 1.8	11.9 ± 0.2	40.4 ± 0.1	38.5 ± 0.4	22.6 ± 0	14 ± 0.4	30.4 ± 0.3	14.3 ± 0.3	21.2 ± 0	40.8 ± 0.1	40.7 ± 0.9
ALA %a	51.8 ± 1.7	13 ± 0.1	50.3 ± 0.3	40.7 ± 0.3	42 ± 3.4	9.8 ± 0.7	40.8 ± 0.8	5 ± 1.1	23.9 ± 0.1	50.2 ± 1.9	41 ± 1.4

^aLA % or ALA % = 100 × ([product] / [product + substrate]).