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Investigation of the incubation times for the samples and the phycoerythrin labeled anti-human IgG detection antibody. (a,b) Four different incubation times (10 min, 20 min, 30 min, and 40 min) were tested in four samples. Depicted are the MFI values of all used H. pylori antigens of two samples. (c) Kinetic of incubation with the detection antibody for 11 min. A measurement was performed approximately every 30 s.
