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. 2017 Oct 11;5(4):26. doi: 10.3390/proteomes5040026

Figure 2.

Figure 2

Heat map representation of identified Salmonella proteins and corresponding label-free quantification (LFQ) intensities when analyzing complex host/pathogen peptide mixtures. Complex peptide mixtures obtained from a trypsin digested total Salmonella protein lysate (S) dilution series (as indicated in the Figure) in protein lysates of human HeLa cells (H) were analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and the protein abundance quantified using label-free quantification (LFQ) [24]. In the heat map, the red and green color indicates high and low LFQ intensities (log2 (LFQ)) as shown by the color scale bar in log2 LFQ), respectively and white indicates that the protein was not identified in the corresponding experimental setup analyzed. In the upper part of the figure, the bar chart shows the number of proteins identified in every condition. The precise number of Salmonella proteins identified was 1600 (S), 1391 (S:H 1:1), 1120 (S:H 1:3), 950 (S:H 1:9), 736 (S:H 1:19), 553 (S:H 1:49), 427 (S:H 1:99), 328 (S:H 1:399), 295 (S:H 1:999).