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Multicycle growth kinetics of parent NDV strain F and recombinant viruses rNDV-F/VP2 and rNDVF in Vero cells. Monolayer of Vero cells were infected with each of the viruses at a multiplicity of infection (MOI) of 0.01 and supernatant collected and replaced with an equal volume of fresh medium at 12 h interval until 72 h, and viral titers were determined by limiting dilution assay and calculated as TCID50 by Reed and Muench method.
