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. 2017 Nov 4;5(4):41. doi: 10.3390/vaccines5040041

Figure 2.

Figure 2

pH-responsive properties of DLPC/deoxycholic acid micelles. (a) Time courses of pyranine fluorescence for pyranine-loaded EYPC liposomes after addition of DLPC/deoxycholic acid micelles at pH 7.4 (red) or 5.0 (blue). Triton-X100 was added to dissociate the liposomal membrane completely. (b) Comparison of membrane disruptive activity for pyranine-loaded EYPC liposomes by addition of DOPE/CHEMS (green), DOPE/oleic acid (red) and DLPC/deoxycholic acid micelles (blue) at various pH. (c) Lipid mixing assay using EYPC liposomes containing Rh-PE/NBD-PE and various vehicles. Ratios of fluorescence intensity at 530 nm and 580 nm, which indicate a decrease of FRET by lipid mixing, are shown as a function of pH. Methanol was used to achieve complete lipid mixing.