Skip to main content
. 2017 Nov 8;5(4):42. doi: 10.3390/vaccines5040042

Figure 2.

Figure 2

Rac1 localizes to ruffles during ASFV entry. Vero cells were transfected with EGFP-Rac-1 plasmid expression for 16 h and synchronously infected (adsorption period of 90 min at 4 °C) at an multiplicity of infection (MOI) of 10. Cells were fixed and incubated with Topro-3 and monoclonal antibody anti-p72 17LD3 to visualize DNA and viral particles, respectively; samples were processed by CLSM (x63 magnification). Images are representative of three independent experiments (images from D. Pérez-Núñez, E. G. Sanchez and Y. Revilla).