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. 2017 Dec 6;5(4):47. doi: 10.3390/vaccines5040047

Figure 2.

Figure 2

Sublingual immunization with Mtb proteins Ag85B and ESAT-6 in the presence of α-GalCer adjuvant induces CD4 and CD8 T cell responses. C57BL/6 female mice were immunized as in Figure 1 and cells isolated from lung, spleen and cervical lymph node(CLN) were analyzed by intracellular cytokine flow cytometry for CD8 and CD4 T cell responses specific to the Mtb proteins Ag85B and ESAT-6. Flow cytometry gating strategy for detecting the different lymphocyte subsets expressing the cytokines analyzed (panel (A)). Data for the CD8 T cells positive for CD107a and IFN-γ (panels (B–E)), and CD4 T cell for IFN-γ and IL2 (panels (F–I)). Significant responses (*, p < 0.05) between groups vaccinated in the presence and absence of α-GalCer (w/aGC and w/o aGC, respectively) were noted. The experiment was performed using 5 mice/group and repeated 2 times.