Figure 6.

IL-4 treatment increases the area of staining for βIII-tubulin in the caudal part of the injured spinal cord. Distribution of βIII-tubulin positive area along the rostrocaudal axis of the spinal cord (A); Quantification of βIII-tubulin⁺ area in the whole spinal cord did not reveal any differences between saline or IL-4 treated rats (B) however a significant increase of βIII-tubulin area was observed in the caudal (G) area of the spinal cord of IL-4-treated rats; In the epicenter (F) and in the rostral (E) area no differences were observed. Schematic and low-magnification photomicrograph indicating areas where the analyses were performed (dashed lines) (C); Representative images of positive staining of neuronal cytoskeleton of saline (D) and IL-4 treated (H) group. Values shown as mean ± SEM. *** p < 0.001. Scale bar = 100 µm.