Table 2.
Root proteins decreased by more than two-fold at low (4 μM) P in contrasting soybean genotypes.
| Spot ID | Accession Number | Description | Taxonomic Database | E | Mr (kDa) | pI | Mascot Score | #P | C(%) |
|---|---|---|---|---|---|---|---|---|---|
| Carbohydrate metabolic process | |||||||||
| 1a | XP_003547334.1 | Predicted: Triosephosphate isomerase isoform X1 | Glycine max | 5.3 × 10−6 | 27.4 | 5.87 | 120 | 9/11 | 36 |
| 2a | XP_003531895.1 | Predicted: 6-Phosphogluconate dehydrogenase, decarboxylating 3-like isoform 1 | Glycine max | 5.3 × 10−6 | 53.8 | 6.11 | 120 | 11/9 | 31 |
| 3a | AAS18240.1 | Enolase | Glycine max | 0.017 | 48.0 | 5.31 | 85 | 9/11 | 23 |
| 4a | OQU84669.1 | Hypothetical protein | Sorghum bicolor | 0.028 | 4.0 | 10.04 | 83 | 4/16 | 94 |
| Protein metabolic process | |||||||||
| 5a | XP_003552094.1 | Predicted: Probable mitochondrial-processing peptidase subunit β-like | Glycine max | 1.1 × 10−7 | 58.8 | 6.49 | 137 | 12/8 | 27 |
| 6a | OMO96428.1 | Hypothetical protein | Glycine max | 0.24 | 85.7 | 5.22 | 73 | 9/11 | 11 |
| Biosynthetic process | |||||||||
| 7a | NP_001235794.1 | Methionine synthase | Glycine max | 3.3 × 10−11 | 84.4 | 5.93 | 172 | 15/5 | 25 |
| 8a | NP_001235219.1 | Chalcone isomerase A | Glycine max | 0.0046 | 23.3 | 6.23 | 91 | 7/13 | 41 |
| 9b | NP_001238531.2 | Cytosolic glutamine synthetase | Glycine max | 4.2 × 10−5 | 39.1 | 5.48 | 111 | 9/11 | 31 |
| 10b | XP_003546821.1 | Predicted: Phosphoglycerate kinase, cytosolic-like | Glycine max | 0.00074 | 42.4 | 5.48 | 98 | 9/11 | 28 |
| 11b | XP_016685735.1 | L10-interacting MYB domain-containing protein-like, isoform X1 | Gossypium hirsutum | 0.14 | 36.0 | 5.96 | 76 | 7/13 | 20 |
| 12a,b | XP_003554033.1 | Predicted: 5-Methytetrahydropteroyltriglutamate-homocysteine methyltransferase 1 isoform 1 | Glycine max | 4.2 × 10−6 | 84.4 | 8.73 | 121 | 12/8 | 23 |
| 13a,b | XP_003529397.1 | Predicted: Ferredoxin-nitrite reductase, chloroplastic-like | Glycine max | 0.036 | 66.5 | 5.97 | 82 | 9/11 | 14 |
| 14a,b | XP_016752752.1 | Uncharacterized: ATP synthase CF0 subunit I | Gossypium hirsutum | 0.29 | 26.4 | 6.31 | 73 | 7/13 | 21 |
| Nucleobase-containing compound metabolic process | |||||||||
| 15a | Gm_SSP8106a | Predicted protein | Ostreococcus lucimarinus | 0.28 | 46.3 | 6.30 | 73 | 6/9 | 19 |
| 16a,b | XP_021735866.1 | Uncharacterized: ATP sulfurylase 2 | Chenopodium quinoa | 2.6 | 92.0 | 5.49 | 63 | 7/13 | 8 |
| Cellular process | |||||||||
| 17b | XP_009141007.1 | Predicted: Tubulin β-7 chain-like | Brassica napus | 0.00017 | 51.2 | 4.73 | 105 | 9/11 | 22 |
| 18b | XP_016580376.1 | Sulfite oxidase | Capsicum annuum | 3.0 | 69.1 | 9.45 | 62 | 6/14 | 11 |
| 19b | OQU86022.1 | Hypothetical: Kinesin heavy chain | Sorghum bicolor | 3.5 | 14.6 | 9.35 | 62 | 5/15 | 48 |
| Generation of precursor metabolites and energy | |||||||||
| 20a | AAA33978.1 | Isocitrate dehydrogenase | Glycine max | 5.3 × 10−9 | 49.5 | 6.13 | 150 | 13/7 | 32 |
| 21a,b | NP_001241237.1 | Uncharacterized protein | Glycine max | 6.6 × 10−9 | 46.4 | 5.87 | 149 | 13/7 | 32 |
| Metabolic process | |||||||||
| 22a | XP_013467839.1 | Pyridoxal-5′-phosphate-dependent enzyme family protein | Medicago truncatula | 0.21 | 22.2 | 7.66 | 74 | 6/14 | 30 |
| Signal transduction | |||||||||
| 23a | XP_006584765.1 | Predicted: Calcineurin B-like protein 2-like | Glycine max | 4.0 | 23.9 | 4.84 | 61 | 5/15 | 22 |
| Nucleotide/Nucleic acid binding | |||||||||
| 24a | XP_009797404.1 | Predicted: Glycine-rich RNA-binding protein 4 | Nicotiana sylvestris | 1.3 | 18.4 | 9.32 | 66 | 5/15 | 31 |
| 25a | NP_001276267.2 | Predicted: Elongation factor Tu, mitochondrial-like | Glycine max | 0.05 | 49.3 | 6.40 | 80 | 8/12 | 23 |
| 26b | XP_016674018.1 | Predicted: Coiled-coil domain-containing protein 22 homolog isoform X2 | Gossypium hirsutum | 2e+2 | 56.1 | 4.93 | 44 | 5/8 | 8 |
| Transport | |||||||||
| 27a | XP_007509415.1 | Unknown protein | Bathycoccus prasinos | 0.86 | 84.2 | 5.04 | 68 | 7/13 | 10 |
| 28a | XP_021753870.1 | Uncharacterized: ATP synthase CF1 α | Chenopodium quinoa | 0.18 | 20.2 | 8.91 | 75 | 5/7 | 19 |
| Protein binding | |||||||||
| 29a | NP_001239642.1 | Glutathione S-transferase | Glycine max | 3.3 × 10−5 | 24.9 | 5.73 | 112 | 7/13 | 36 |
| 30a | XP_021316845.1 | Acyl-binding domain-containing protein 5-like | Sorghum bicolor | 0.19 | 77.1 | 8.85 | 74 | 9/11 | 19 |
| 31a,b | XP_003531506.1 | Predicted: Nitrile-specifier protein 5-like | Glycine max | 3.3 × 10−15 | 36.0 | 5.59 | 212 | 14/6 | 55 |
| Catalytic activity | |||||||||
| 32a | KHN43834.1 | Aldo-keto reductase family 4 member C9-like | Glycine max | 1.3 × 10−9 | 35.0 | 6.40 | 156 | 12/8 | 47 |
| 33a | NP_001340170.1 | Predicted: Alcohol dehydrogenase 1 | Glycine max | 2.6 × 10−5 | 41.6 | 5.97 | 113 | 9/11 | 32 |
| 34a | KHN30856.1 | NADP-dependent alkenal double bond reductase | Glycine max | 0.036 | 38.0 | 5.94 | 82 | 7/13 | 25 |
| 35a | XP_003549946.1 | Predicted: Expansin-like B1-like | Glycine max | 0.00021 | 28.2 | 6.30 | 104 | 7/13 | 43 |
| 36b | KHN16790.1 | Monodehydroascorbate chloroplastic | Glycine max | 2.1 × 10−5 | 52.4 | 8.36 | 144 | 12/8 | 33 |
| 37b | XP_003521330.1 | Heat shock protein 70 | Glycine max | 6.6 × 10−7 | 71.8 | 5.05 | 129 | 12/8 | 26 |
| 38b | NP_001234990.1 | Peroxisomal betaine-aldehyde dehydrogenase | Glycine max | 0.00021 | 55.4 | 5.23 | 104 | 10/10 | 17 |
| 39b | KCW74152.1 | Hypothetical protein | Eucalyptus grandis | 0.046 | 52.8 | 9.73 | 81 | 10/10 | 18 |
| Kinase activity | |||||||||
| 40b | ONH93257.1 | Hypothetical: Serine threonine-protein kinase 19 | Prunus persica | 0.84 | 25.3 | 9.11 | 68 | 7/13 | 25 |
| Transferase activity | |||||||||
| 41b | XP_004968119.1 | Predicted: o-linked n-acetylglucosamine transferase-like | Setaria italica | 0.11 | 50.3 | 10.45 | 77 | 8/12 | 16 |
| Unknown | |||||||||
| 42a | XP_018476189.1 | Predicted: RRP12-like protein | Raphanus sativus | 0.44 | 141.5 | 8.88 | 71 | 9/11 | 11 |
| 43a | XP_016506991.1 | Uncharacterized protein | Nicotiana tabacum | 0.45 | 17.4 | 8.95 | 71 | 6/14 | 25 |
| 44b | XP_011040448.1 | Uncharacterized protein | Populus euphratica | 2.1 | 83.8 | 6.22 | 64 | 7/7 | 6 |
Spot ID corresponds to the protein spots in Supplemental Figure S3A,C. Superscripts a and b denote decreased proteins at low P compared to sufficient P in EC-232019 and EC-113396, respectively. E denotes the expectation value, Mr and pI are monoisotopic mass and calculated isoelectric point, respectively. #P and C denote the number of matched/unmatched peptides and protein sequence coverage, respectively. Proteins with Mascot score > 80 are significant (P < 0.05).