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. 2017 Dec 8;8(12):373. doi: 10.3390/genes8120373

Figure 2.

Figure 2

Spot on lawn “halo” assays for functional SSV1 VP1 mutants. Sulfolobus cultures infected with SSV1 containing various VP1 mutations were spotted on a lawn of uninfected Sulfolobus. The lawn and infected cultures were allowed to grow for 48 hours and then photographed. Letters indicate indicates a spotted culture of Sulfolobus infected with a SSV1 that contains the wild type VP1 gene (A); the VP1 gene from SSV9 (B); a fusion between the N-terminus of VP1 from SSV9 and the C-terminus of VP1 from SSV1 (C) the VP1 gene from SSV2 (D); a glutamine at position 66 in the SSV1 VP1 protein instead of the wild type glutamate (E), or an alanine at position 66 in the SSV1 VP1 protein instead of the wild type glutamate (F); (G) indicates an uninfected control culture spotted on the lawn.