Table 2.
Primers used in this study.
| Product | Forward Primer | Reverse Primer |
|---|---|---|
| SSV1 VP1 deletion | TGA GGG ATG GAA ATC AGT TTA AAG | CAA ACT CCT TAG GAG TCT CAT CC |
| SSV1 VP1 N-terminus deletion | ATG GAA GCA ACC AAC ATA GG (61) and GAA GCA ACC AAC ATA GG (54) | CAA ACT CCT TAG GAG TCT CAT CC |
| SSV1 VP1 aa61–65 deletion | GAA GCA ACC AAC ATA GGC | GGG GTT TGC CTT TGC TAC |
| SSV1 VP1 point mutant (E66A) A | GCA GCA ACC AAC ATA GGC | ACC TTT TGT GAG CTT GGG G |
| SSV1 VP1 point mutant (E66Q) B | CAA GCA ACC AAC ATA GGC | ACC TTT TGT GAG CTT GGG G |
| SSV1 VP1 C | GCcAGAAAGATAGCCTCAC | ACCTTTTGTGAGCTTGGG |
| SSV9 VP1 | GAAGTTTGGTCAAAGTTAAACG | ATCTTTGTAGATTTTATACG |
| SSV2 VP1 | GCCACCAGACTAATGCTAAGC | GTCACGATATATCTTATACGCTATGAC |
| SSV9 VP1 N-terminus | GAAGTTTGGTCAAAGTTAAACG | ACCCCTAGTAAGTTTGGG |
All sequences are written 5′ → 3′, bases in bold type are within the SSV1 ORF; A Highlighted base indicates mismatch generating Glu→ Ala mutation in VP1 protein; B Highlighted base indicates mismatch generating Glu→ Gln mutation in VP1 protein; C Lower case denotes introduced silent restriction endonuclease site. ATG: start codon.