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. 2017 Dec 8;8(12):374. doi: 10.3390/genes8120374

Table 1.

Bacterial strains, plasmids and oligonucleotides used in this study.

Strain, Plasmid or Oligonucleotide Characteristics or Sequence a Reference or Source
Bacterial strains
Bradyrhizobium elkanii
USDA61 Wild-type strain, Polr Keyser b
BErhcJ USDA61 derivative harboring insertion in rhcJ region, Polr, Kmr, Tcr [12]
BE5 Tn5 mutant of USDA61, Polr, Kmr This study
BE53 Tn5 mutant of USDA61, Polr, Kmr This study
BE85 Tn5 mutant of USDA61, Polr, Kmr This study
BE103 Tn5 mutant of USDA61, Polr, Kmr This study
BE168 Tn5 mutant of USDA61, Polr, Kmr This study
BE53S USDA61 derivative containing an insertion of plasmid pSUPSCAKm:int innB, Polr, Kmr This study
Escherichia coli
HB101 recA, hsdR, hsdM, pro, Smr Invitrogen, Carlsbad, CA, USA
S17-1 pro recA RP4-2(Tcs:Mu) (Kms:Tn7); Mob+ [20]
DH10B Cloning strain Invitrogen
Plasmids
pUTKm Transposon delivery vector; Apr, Kmr [21]
pRK2013 Helper plasmid, ColE1 replicon carrying RK2 transfer genes; Kmr, tra [22]
pSUPSCAKm Derivative of pSUPPOL2SCA [23] with a kanamycin resistance gene in the DraI site, oriT of RP4, Tcr, Kmr This study
pSUPSCAKm:int innB pSUPSCAKm carrying a 0.5-kb DNA fragment containing internal sequence of innB, Kmr This study
Oligonucleotides
Linker 1 5′-TTTCTGCTCGAATTCAAGCTTCTAACGATGTACGGGGACACATG-3′ [24]
Linker 2 5′-TGTCCCCGTACATCGTTAGAACTACTCGTACCATCCACAT-3′ [24]
Y-linker primer 5′-CTGCTCGAATTCAAGCTTCT-3′ [24]
Tn5 primer 5′-GGCCAGATCTGATCAAGAGA-3′ [24]
Psuppol-F 5′-ATAAACCAGCCAGCCGGAA-3′ This study
Psuppol-R 5′-TTCTGACAACGATCGGAGGA-3′ This study
BE5-F 5′-TCATGCAGGTGAATGTCGAT-3′ This study
BE5-R 5′-CTATCCGCAGGAGTTGAACG-3′ This study
BE53-F 5′-AGATTGATGTTGCCGAGGAC-3′ This study
BE53-R 5′-TGAAAAAGCTCCGTGAGGTC -3′ This study
BE85-F 5′-GCGCGGATATTGACATTGAT-3′ This study
BE85-R 5′-AGGCCGTCGATCTCTATCAC-3′ This study
BE103-F 5′-ACAAGAAGATGTCGGCCAAG-3′ This study
BE103-R 5′-TGCTCGCAGAATACAACTGC-3′ This study
BE168-F 5′-TCGAAAGCGCACTAGATTGA-3′ This study
BE168-R 5′-AGCCGTAAATATCGGACAGC-3′ This study
InnBXbaI-intF 5′-CGGTGGCGGCGGCCGCTCTAGAAAAATGCGCAACTGGAAGAT-3′ This study
InnBEcoRI-intR 5′-CGATAAGCTTGATATCGAATTCATCTGCTCACCAAGCCAATC-3′ This study

a Polr: Polymyxin resistant; Kmr: Kanamycin resistant; Smr: Streptomycin resistant; Spr: Spectinomycin resistant; Tcr: Tetracycline resistant; Apr: Ampicillin resistant; inn: incompatible nodulation. b United States Department of Agriculture, Beltsville, MD, USA.