. 2017 Dec 8;8(12):374. doi: 10.3390/genes8120374

Table 2.

Results of BLAST and tts box searches of Tn5-flanking sequences.

		Gene with Tn5 Insertion
Strains	Nodulation on Rj4 Soybean ^a	Designated Symbol/GenBank Accession No. ^b	Length (bp)	Deduced Gene Products	tts Box ^c	Strain and Locus Tag/Gene (GenBank Accession No., % Identity by BLASTP Analysis)
BE5	+	innA/KX499540	1212	Cytosine deaminase	-	Bradyrhizobium pachyrhizi AOQ73_15990 (KRQ04415.1, 95%), Afipia clevelandensis ATCC 49720 HMPREF9696_04200 (EKS31979.1, 77%), Agrobacterium rhizogenes CN09_18315 (KEA04550.1, 61%), Bradyrhizobium diazoefficiens NK6 NK6_7958 (BAR61109.1, 59%), Bradyrhizobium sp. BTAi1 BBta_7204 (ABQ39084.1, 59%), Nitrosospira sp. NpAV SQ11_05400 (KIO49569.1, 58%), Mesorhizobium loti NZP2014 A8146_24615 (OBQ73313.1, 56%), Azorhizobium caulinodans ORS571 AZC_1945 (BAF87943.1, 49%), Sinorhizobium meliloti 1021 SMc02420 (CAC47175.1, 40%), Pseudomonas fluorescens C3 VC34_22545 (KJZ38903.1, 43%)
BE53	-	innB/KX499541	2280	Hypothetical protein	+	Bradyrhizobium yuanmingense BR3267 AOQ72_03805 (KRP85897.1, 73%), B. japonicum USDA6 BJ6T_78540 (BAL13100.1, 71%), B. diazoefficiens USDA110 blr1998 (BAC47263.1, 70%), B. japonicum USDA 6 BJ6T_78550 (BAL13101.1, 73%), B. japonicum USDA 6 BJ6T_78530 (BAL13099.1, 66%), B. diazoefficiens USDA110 bll1877 (BAC47142.1, 46%), M. loti MAFF303099 mlr6327 (BAB52639.1, 45%)
BE85	+	innC/KX499542	504	TerB family tellurite resistance protein	-	Bradyrhizobium erythrophlei MT12 SAMN05444164_5326 (SED64040.1, 97%), B. diazoefficiens NK6 NK6_3937 (BAR57108.1, 81%), B. japonicum Is-34 MA20_25200 (KGT77838.1, 80%), B. japonicum USDA6 BJ6T_05250 (BAL05822.1, 79%), A. clevelandensis ATCC 49720 HMPREF9696_03100 (EKS33980.1, 77%), Proteobacteria bacterium SG_bin9 A4S14_11410 (OQW55861.1, 76%), Rhodopseudomonas sp. AAP120 IP86_13420 (KPF97560.1, 73%), Nitrobacter hamburgensis X14 Nham_0605 (ABE61494.1, 72%)
BE103	+	innD/KX499543	1089	ABC transporter substrate-binding protein	-	B. pachyrhizi BR3262 AOQ73_18465 (KRQ01332.1, 99%), B. diazoefficiens USDA110 blr7816 (BAC53081.1, 87%), Afipia sp. P52-10 X566_18245 (ETR74761.1, 62%), Rhodopseudomonas palustris BisB5 RPD_2049 (ABE39284.1, 61%), Azospirillum brasilense Sp7 AMK58_19775 (ALJ37675.1, 61%), B. japonicum USDA6 BJ6T_61450 (BAL11399.1, 60%), Bosea vaviloviae SD260 AE618_12875 (KPH80633.1, 61%), Agrobacterium tumefaciens KCJ17 A7J57_24755 (OAE45812.1, 59%), B. diazoefficiens USDA110 blr3743 (BAC49008.1, 59%)
BE168	+	innE/KX499544	2292	GTP pyrophosphokinase	-	B. pachyrhizi BR3262 AOQ73_36790 (KRP86030.1, 99%), Bradyrhizobium jicamae PAC68 CQ12_18875(KRQ98494.1, 94%), B. diazoefficiens USDA110 bll5065 (BAC50330.1, 88%), B. japonicum USDA 6 BJ6T_46480 (BAL09914.1, 87%), R. palustris BisB18 RPC_2635 (ABD88185.1, 87%), S. meliloti 1021 SMc02659 (CAC45644.1, 60%), A. tumefaciens KCJ1736 A7J57_18100 (OAE38384.1, 60%), A. brasilense Sp7 AMK58_17855 (ALJ37338.1, 52%), Frankia alni ACN14a FRAAL2148 (CAJ60797.1, 40%)

^a Nodulation on G. max cv. BACR2 (Rj4Rj4) soybean; +: Positive; −: Negative. ^b inn: incompatible nodulation. ^c Presence of tts box in upstream regions of the genes; +: Present; −: Absent.