Table 1.
Quantitative reverse transcription PCR (qRT-PCR) and RNA-sequencing (RNA-seq) analysis of genes with induced expression under nitrogen-starved conditions.
| Locus ID 1 | Description 1 | Gene Name | FC (S vs. N) 2 | FC (S vs. N) 3 |
|---|---|---|---|---|
| Bphy_0257 | Ammonium transporter | amtB | 19.7 ± 4.6 | 10.9 |
| Bphy_0326 | RNA polymerase factor σ54 | rpoN | 1.4 ± 0.1 | 0.7 |
| Bphy_1479 | Nitrogen metabolism transcriptional regulator | ntrC | 8.8 ± 1.2 | 9.7 |
| Bphy_1481 | Glutamine synthetase, type I | glnA | 4.0 ± 0.8 | 1.9 |
1 Locus identifier and description is given according to the GenBank files (NC_010622.1, NC_010623.1, NC_010625.1, NC_010627.1); 2 fold change (FC) in qRT-PCR expression by comparing the wild-type strain grown under nitrogen-limited (S) and nitrogen-replete conditions (N); 3 fold change (FC) determined by DESeq analysis comparing the transcriptome profile of cells grown under nitrogen-limited (S) and nitrogen-replete conditions (N).