Table 1.
List of PCR primers used in the study.
| Primer Name | Coverage | Sequence (5′-3′) | Size (bp) | Annealing Temperature (°C) |
|---|---|---|---|---|
| DKK1-F1 | GCAAAGCGACACTCCTCC | 955 | 59.0 | |
| DKK1-R1 | GCTCTTACACCCCAGATTTTCGG | |||
| DKK1-F2 | chr22:6668693-6670458 | ATGAAACCGAACTCTTGACTTA | 1771 | 58.6 |
| DKK1-R2 | AACTTGATTAGGCAGACACG | |||
| DKK1-F3 | chr22:6667603-6668709 | GTCTGCYTAATCAAGTTCATCTAC | 1107 | 56.2 |
| DKK1-R3 | GGTTCTTGATV4GCGTTGGAAT | |||
| DKK1-F4 | chr22:6665566-6667623 | ATTCCAACGCB1ATCAAGAACC | 2100 | 56.2 |
| DKK1-R4 | CAGR2CCTTCY3CCRCAGTAACA | |||
| DKK1-F5 | chr22:6665250-6665656 | TCTCAAGGAAGGTCAAGTGT | 407 | 52.0 |
| DKK1-R5 | GGTTGCATTTACAGGTAAGT | |||
| DKK1-F6 | chr22:6663304-6665269 | ACTTACCTGTAAATGCAACC | 1966 | 46.9 |
| DKK1-R6 | TTATCCTACAACTATATCAGCAC |
F: forward primer; R: reverse primer. Degenerate bases: B1, C/G/T; R2, A/G; Y3, C/T; V4, A/C/G.