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. 2017 Dec 12;9(12):1349. doi: 10.3390/nu9121349

Figure 3.

Figure 3

Effect of combining cell and bacterial culture medium on the viability and TEER of Caco-2 cells in the apical anaerobic co-culture model. The apical compartment of Caco-2 cell monolayers were exposed to non-supplemented anaerobic M199 TEER medium (0% BHI), or M199 TEER medium supplemented with increasing concentrations of anaerobic BHI medium (25%, 50%, 75%, or 100% BHI). (a) Viability (mean ± SEM; n = 20) of Caco-2 cells after 12 h incubation. Neutral red absorbance was normalized by adjusting the 0% BHI exposed cells to 1. Viability of the Caco-2 cells was unchanged as a consequence of differing culture medium composition (p = 0.07). (b) Mean (±SEM; n = 20) change in TEER as a percentage of initial TEER across Caco-2 cell monolayers over 12 h for each medium. There was a significant interaction between the time and the BHI concentration on the change in TEER (p < 0.01). Treatments that do not share the same letters are significantly different (p < 0.05).