Figure 4.

Viability of the three F. prausnitzii strains in a cell culture medium supplemented with bacterial culture medium. (a) In Hungate culture tubes. The three F. prausnitzii strains in stationary phase were resuspended in anaerobic M199 TEER: BHI (1:1) (referred to as 50% BHI) and incubated anaerobically at 37 °C. The graph shows the mean (±SEM; n = 3) log₁₀(CFU/mL) of the three F. prausnitzii strains before and after 12 h of incubation in 50% BHI. * Mean log₁₀(CFU/mL) differ between 0 and 12 h at p < 0.05. (b) In the apical anaerobic co-culture model with Caco-2 cells. The three F. prausnitzii strains were co-cultured with Caco-2 cells using two different media on the apical side of the Caco-2 cell monolayer (25% and 50% BHI). The graph shows the mean (±SEM; n = 4 and 8 for time 0 and 12 h, respectively) log₁₀(CFU/mL) of the bacteria at 0 and 12 h of incubation with Caco-2 cells in the co-culture model. Mean log₁₀(CFU/mL) differ between 0 and 12 h at * p < 0.05 and ** p < 0.01.