Skip to main content
. 2017 Dec 15;9(12):1363. doi: 10.3390/nu9121363

Table 1.

Primers used to analyze mRNA expression by real-time PCR.

Accession No. Gene Sequence Product Size (bp) Annealing Temp (°C) Cycles
NM.031144 β-actin 5′-CACCACAGCTGAGAGGGAAA-3′ 3′-TGGACAGTGAGGCCAGGATA-5′ 472 66 40
NM.012611 iNOS 5′-AGGAGATGGTCCGCAAGGGA-3′ 3′-TCGTCGGCCAGCTCTTTCTG-5′ 120 66 40
NM.022852 pdx-1 5′-GGTATAGCCAGCGAGATGCT-3′ 3′-TCAGTTGGGAGCCTGATTCT-5′ 153 66 40
M57441 MCP-1 5′-CTTCTGGGCCTGTTGTTCA-3′ 3′-CCAGCCGACTCATTGGGATCA-5′ 127 66 40
NM.013129 IL-15 5′-TGCAATGAACTGCTTTCTCCTGGAA-3′ 3′-GCTCCTCACATTCCTTGCAGCC-5′ 158 66 40
NM.139089 IP-10 5′-TCGTTCTCTGCCTCGTGCTGC-3′ 3′-ATGGCCCTGGGTCTCAGCGT-5′ 115 66 40
AF443192 spXBP-1 5′-CGCTTGGGAATGGACACGCT-3′ 3′-CTGCACCTGCTGCGGACTCA-5′ 106 66 40
NM.001110823 SERCA2b 5′-CAAACAAACCGAGCCGGACG-3′ 3′-AACAACAGGCGTCATGGGGA-5′ 122 66 40
U36994 CHOP 5′-TGCTGAAGAGAACGAGCGGC-3′ 3′-GGTGCAGACTGACCATGCGG-5′ 104 66 40