Skip to main content
. 2017 Dec 18;9(12):1370. doi: 10.3390/nu9121370

Figure 3.

Figure 3

miR-132 regulates PA-induced NLRP3 inflammasome activation. (A–F) THP-1 cells were transfected with a mimic negative control, mimic, inhibitor negative control, or inhibitor of miR-132 at 20 nM for 48 h before being treated with 200 μM PA for 12 h; (A,C) The mRNA levels of NLRP3, caspase-1, IL-18, and IL-1β were examined by quantitative RT-PCR; (B,D) Cell lysates and supernatants were subjected to Western blot analysis using antibodies specific for NLRP3, caspase-1, IL-18, IL-1β, and β-actin. β-Actin was used as a loading control; (E,F) The levels of caspase-1 and IL-1β in the culture supernatants were determined by ELISA; (G–I) Primary murine macrophages were transfected with inhibitor negative control or inhibitor of miR-132 at 20 nM for 48 h before being treated with 200 μM PA for 12 h; (G) The mRNA levels of NLRP3, caspase-1, IL-18, and IL-1β were determined using quantitative RT-PCR; (H,I) The levels of caspase-1 and IL-1β in the culture supernatants were determined by ELISA. # p < 0.05 compared with the mimic or inhibitor negative control in PA-untreated cells, and * p < 0.05 compared with the mimic or inhibitor negative control in PA-treated cells.