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. 2017 Dec 18;9(12):1370. doi: 10.3390/nu9121370

Figure 6.

Figure 6

The silencing of FOXO3 suppresses PA-induced NLRP3 inflammasome activation. (A–E) THP-1 cells were transfected with 20 nM control siRNA or FOXO3 siRNA for 48 h and then treated with PA (200 μM) for 12 h; (A) The FOXO3 siRNA transfection efficiency was measured by Western blot analysis; (B) The mRNA levels of NLRP3, caspase-1, IL-18, and IL-1β were examined by quantitative RT-PCR; (C) Cell lysates and supernatants were subjected to Western blot analysis using antibodies specific for NLRP3, caspase-1, IL-18, IL-1β, and β-actin. β-Actin was used as a loading control; (D,E) The levels of caspase-1 and IL-1β in the culture supernatants were determined by ELISA; (F–H) Primary murine macrophages were transfected with 20 nM control siRNA or FOXO3 siRNA for 48 h and then treated with PA (200 μM) for 12 h; (F) The mRNA levels of NLRP3, caspase-1, IL-18, and IL-1β were examined by quantitative RT-PCR; (G,H) The levels of caspase-1 and IL-1β in the culture supernatants were determined by ELISA. # p < 0.05 compared with the control siRNA in PA-untreated cells, and * p < 0.05 compared with the control siRNA in PA-treated cells.