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. 2018 Jan 2;215(1):303–318. doi: 10.1084/jem.20160217

Table 1. Kinetic constants of CXCL12, 3S, and BoxA binding to CXCR4.

Ligand Ka Kd KD
1/Ms 1/s nM
CXCL12 1.5 × 105 5.83 × 10−2 76.9
3S 6.81 × 105 1.04 × 10−3 6.4
BoxA 5.75 × 106 2.3 × 10−2 6.7

Curves derived from binding of CXCL12, 3S, and BoxA to immobilized lentiviral particles with membrane-bound CXCR4 were used to generate kinetic constants (Ka, Kd, KD) by using a simple one-site interaction model with BIA Evaluation 4.1 software (GE Healthcare). Data are representative of three independent experiments.