Table 1. Kinetic constants of CXCL12, 3S, and BoxA binding to CXCR4.
Ligand | Ka | Kd | KD |
---|---|---|---|
1/Ms | 1/s | nM | |
CXCL12 | 1.5 × 105 | 5.83 × 10−2 | 76.9 |
3S | 6.81 × 105 | 1.04 × 10−3 | 6.4 |
BoxA | 5.75 × 106 | 2.3 × 10−2 | 6.7 |
Curves derived from binding of CXCL12, 3S, and BoxA to immobilized lentiviral particles with membrane-bound CXCR4 were used to generate kinetic constants (Ka, Kd, KD) by using a simple one-site interaction model with BIA Evaluation 4.1 software (GE Healthcare). Data are representative of three independent experiments.