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. 2018 Jan 2;215(1):337–355. doi: 10.1084/jem.20170457

Figure 5.

Figure 5.

T-bet in Tfh cells is required for proper GC B cell output after viral infection. T-bet+/+, T-bet+/−, or T-bet−/− Thy1.1+ Stg CD4+ T cells were transferred to TCR-β−/− mice. Spleens were harvested 13 d p.i. (A) Number of T-bet+/+, T-bet+/−, or T-bet−/− donor cells that differentiated into Tfh cells in spleens of recipients. (B) Representative flow cytometry plots of CD4−B220+IgDloCD95hiGL-7hi GC B cells in recipients of T-bet+/+, T-bet+/−, or T-bet−/− donor cells with cell percentages and numbers. (C) Representative flow cytometry plots of intracellular IgG1 and IgG2c staining of GC B cells with percentages of isotype-staining cells. (D) Anti-LCMV IgG2c and IgG1 antibodies in sera of recipient mice. (E) Splenic B cell follicles of TCR-β−/− recipient mice stained with anti-IgD, anti-CD4, and peanut agglutinin (PNA), with the numbers of T cells per GC size (bottom left) and GC sizes (bottom right) quantified. (F and G) Total splenocytes from mice 12 d p.i., co-cultured with CXCL13, CXCL12, or media to assess percentages of Tfh cells migrating to each (F and G) or stained for CXCR4 (G). (H) Staining of Tfh cells in recipient spleens for CD40L. Data are representative of three experiments with three to five recipients per group. *, P < 0.05; **, P < 0.01; ***, P < 0.001 by Student’s t test. Error bars represent SEM.