Table 1. Absolute cell numbers in each EHT phenotypic subset in the AGM as determined by flow cytometry.
Gata2 genotype | Stage (sp) | CD31+ | CD31+ ckit+V− | CD31+ ckit−V+ | CD31+ ckit+Vmed | CD31+ ckit+Vhi | Number of experiments (embryos) |
---|---|---|---|---|---|---|---|
+/+ | E10 (28–36) | 10,860 ± 1,704 | 1,257 ± 264 | 1,076 ± 153 | 616 ± 223 | 64 ± 16 | n = 5 (25) |
+/− | E10 (30–36) | 11,664 ± 1,992 | 1,049 ± 258 | 780 ± 179 | 186 ± 60 | 32 ± 6 | n = 3 (19) |