Figure 8.

Milk-borne IgG-IC induces allergen-specific T reg cells via MLN CD11c⁺ DCs. (A and B) Flow cytometric analysis (A) and frequencies (B) of CD4⁺DO11.10⁺Foxp3^EGFP+ T reg cells. MLN CD11c⁺ cells were isolated from naive WT mice and cultured with CD4⁺DO11.10⁺Foxp3^EGFP- cells in the presence or absence of breast milk. (C and D) Flow cytometric analysis (C) and frequencies (D) of CD4⁺DO11.10⁺Foxp3^EGFP+ T reg cells. MLN CD11c⁺ cells were isolated from offspring of SAL or OVA mothers and cultured with CD4⁺DO11.10⁺Foxp3^EGFP- cells without exogenous allergens. (E–L) Adoptive transfer of MLN CD11c⁺ cells from offspring of SAL or OVA mothers. Experimental protocol (E), OVA-IgE (F), core body temperature change (G), serum mMCP1 levels (H), jejunal mast cell frequencies (I) and numbers (J), and flow cytometric analysis of CD4⁺DO11.10⁺Foxp3^EGFP+ T reg cells in MLN (K) and jejunum (L) of recipients. Representative plots from two independent experiments shown (A and C). Numbers indicate percentages (A and C). Groups of animals were compared using nonparametric one-way ANOVA (B) and the Mann-Whitney U test (D and F–L). Data are mean ± SEM of two independent experiments (B and D) or representative of two independent experiments (F–L). *, P < 0.05; **, P < 0.01; ns, not significant. SAL, saline.