Table 4.
IPA Upstream regulators associated with the genes selected from literature analysis and co-expressed genes.
| Upstream Regulator | Molecule Type | P-Value | Target Molecules in Integrated Dataset |
|---|---|---|---|
| CDKN1A | Kinase | 6.31E-09 | BIRC5,CCNA2,FOXM1,KIAA0101,KRT18, MMP1,TOP2A (7) |
| AR | ligand-dependent nuclear receptor | 7.80E-09 | BIRC5,CCNA2,KIF2C,MUC1,NUSAP1, PGR,PIGR,TPX2,UBE2C,ZWINT (10) |
| ERBB2 | Kinase | 1.38E-08 | ASPM,BIRC5,CCNA2,CCNB2,MKI67, MMP1,MUC1,TOP2A,VEGFC,ZWINT (10) |
| FOXO1 | transcription regulator | 3.86E-08 | ASPM,BIRC5,CCNB2,DEPDC1, DLGAP5,MMP1,NUSAP1 (7) |
| TNF | Cytokine | 2.30E-07 | ADIPOQ,BIRC5,ELF3,FST,HLA-DRB4, MMP1,MT-CO2,MUC1,PIGR,PPP2R1B,RRM2, VCAM1,VEGFC (13) |
| FOXM1 | transcription regulator | 1.70E-06 | AURKB,BIRC5,CCNA2,FOXM1, GTSE1 (5) |
| estrogen receptor | Group | 5.25E-06 | KRT18,KRT19,MMP1,MT-CO2, MUC1,PGR,PRLR,VEGFC (8) |
| ESR1 | ligand-dependent nuclear receptor | 7.09E-07 | ASPM,BIRC5,CCNA2,FST,KRT19, MKI67,MMP1,PDZK1,PGR,PRLR,RAMP3 (11) |
| ESR2 | ligand-dependent nuclear receptor | 5.67E-04 | BIRC5,MMP1,PGR (3) |
| LGALS3 | Other | 1.70E-06 | CCNB2,KRT18,KRT19,MUC1,VCAM1 (5) |
| TP53 | transcription regulator | 3.06E-04 | AURKB,BIRC5,CCNA2,HMMR, KIAA0101,RRM2,TOP2A,TPX2,UBE2C (9) |
P-value measures whether there is a statistically significant overlap between dataset genes and the downstream targets of a regulator. The number of different proteins that each upstream regulator has been reported to be associated with is indicated in brackets. The top genes differentially expressed in metastasis (Table 2) are highlighted in red. In bold are TFs that may be responsible for regulation of suggested prognostic marker, RRM2.