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ShcA is upregulated in human proteinuric kidney diseases. Representative confocal images after dual staining for ShcA (red) and nephrin (green) on kidney biopsies from control individuals (n=3) or patients with FSGS (n=4) or MCD (n=3). ShcA is prominently upregulated in FSGS and MCD compared with control biopsies, and is colocalized with nephrin. Biopsies shown were immunostained in parallel, and imaged at the same gain and exposure. Scale bar, 20 μm.
