Table 2.

Genes and primers used in the study

RNA target gene	Primer sequences(5′–3′)	PCR Product		Gene bank Assess. No
		Annealing Temperature	Amplicon (bp)
GAPDH				NM_204305.1
Forward	TGCTGCCCAGAACATCAT	61 °C	199
Reverse	ATCAGCAGCAGCCTTCAC
ACTB				NM_205518.1
Forward	GTCCACCTTCCAGCAGATGT	60 °C	169
Reverse	ATAAAGCCATGCCAATCTCG
NF-kB				M86930.1
Forward	TTGCTGCTGGAGTTGATGTC	60 °C	167
Reverse	TTGCTGCTGGAGTTGATGTC
TNF-α				NM_204267.1
Forward	TTCAGATGAGTTGCCCTTCC	59 °C	150
Reverse	TCAGAGCATCAACGCAAAAG

GAPDH Glyceraldehyde-3-phosphate dehydrogenase, ACTB Actin beta, NF-kB nuclear factor kappa B, and TNF-α Tumor necrosis factor alpha