Fig. 4.

T. gondii-induced NLRP3 inflammasome activation inhibits intracellular parasite proliferation. a FHs 74 Int cells were transfected with NLRP3, Casp-1, ASC or control siRNAs and then infected with T. gondii at an MOI of 10 for 2 h. Cells were fixed and stained with Texas Red-X phalloidin to label F-actin (red), and the nuclei were stained with DAPI (blue). The number of T. gondii-infected cells and the total number of cells were counted, and the T. gondii infection rates were calculated. b siRNA-transfected FHs 74 Int cells were infected with T. gondii at an MOI of 10 for 8 h. Cells were fixed and stained with Texas Red-X phalloidin and DAPI. Intracellular parasites were revealed by fluorescence microscopy. All data shown are representative of three independent experiments. Scale-bars: 20 μm