Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 2;217(1):329–346. doi: 10.1083/jcb.201702179

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Naufer et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 5. — Loss of PtdIns(3)P is correlated with the acidification of distal tPCs. Evolution of p40PX-GFP and pHrodo fluorescence in growing tPCs. RAW macrophages expressing p40PX-GFP (top, green; middle, white) were challenged with pHrodo-conjugated filamentous bacteria and imaged live by simultaneous acquisition of both fluorescence emissions. Micrographs are selected frames from Video 2. pHrodo fluorescence is shown in red in the top row of micrographs and in false-rainbow palette in bottom one. Blue in the rainbow palette indicates neutral pH and red indicates acidic pH. Brackets in the panels from the left indicate position of external filamentous bacteria, detected in bright light field. Arrowheads in lower panels point to the distal tip of the tPCs. Asterisks indicate bacterial-associated pHrodo fluorescence extracted into cytoplasmic vesicles as the tPCs remodel during internalization. Bars, 5 µm.