Table 1. List of Gateway compatible primers used for PCR amplification.
Primers | Sequences |
---|---|
PX-GFP-Gtwy-Forward | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGAAGGAGATAGAACCATGGCTGTGGCCCAGCAGCTG-3′ |
PX-GFP-Gtwy-Reverse | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCCTTGTACAGCTCGTCCATGCC-3′ |
GFP-2FYVE-Gtwy-Forward | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGAAGGAGATAGAACCATGGTGAGCAAGGGCGAGGAG-3′ |
GFP-2FYVE-Gtwy-Reverse | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTATCCTTGCAAGTCATTGAA-3′ |
GFP-Gtwy-Forward | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGAAGGAGATAGAACCATGGTGAGCAAGGGCGAGGAG-3′ |
GFP-Gtwy-Reverse | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCCTTGTACAGCTCGTCCATGCC-3′ |