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. 2018 Jan 2;217(1):211–230. doi: 10.1083/jcb.201704071

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© 2018 Izadi et al.

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Figure 2. — Cobl-like is a brain-enriched protein with neuronal and dendritic localization. (A) Anti–Cobl-like immunoblotting of different mouse tissues. (B and C) Immunohistological analyses of sagittal sections of the hippocampus of adult mice with anti–Cobl-like in combination with the glia marker anti–glial fibrillary acidic protein (GFAP; B) and the neuronal and dendritic marker anti-MAP2 (C), respectively. (D and E) In situ hybridizations of sagittal sections of adult murine brain with a probe against Cobl-like mRNA (Cobl-like antisense) and a negative control probe (sense). Bars: (B and C) 100 µm; (D and E) 200 µm. (F) Cobl-like RT-PCR analyses of different brain regions. GAPDH, control. Rev. Tr. + and −, with and without reverse transcription.