Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 2;217(1):283–298. doi: 10.1083/jcb.201705214

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Thomas et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Figure 7. — TRAPPIII mediates Golgi trafficking. (A) The PSN of TRS85, showing all genes with similar genetic interactions, using a similarity cutoff of 0.4. Note that Uso1 and the COG complex mediate vesicle tethering at the Golgi complex and are known effectors of Ypt1, which is activated by TRAPPIII. (B) Western blot for Kar2 of TCA-precipitated cell and media fractions under normal growth conditions showing aberrant Kar2 secretion in trs85Δ cells but not atg1Δ or atg13Δ autophagy mutants. (C) Western blot comparing Kar2 secretion in trs85Δ and ypt1-3 mutants with that of get1Δ cells, a mutant previously shown to significantly disrupt ER–Golgi transport (Schuldiner et al., 2005). (D) Western blot comparing Kar2 secretion in a trs85Δ mutant with that of snx4Δ and tlg2Δ endosome-recycling mutants or vps17Δ and vps21Δ VPS mutants. Blots are representative of three independent experiments.