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. 2018 Jan 2;217(1):107–116. doi: 10.1083/jcb.201706103

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© 2018 Huang et al.

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Figure 5. — Effects of BuGZ phase transition and binding to AurA on kinase activity. (A) Purified xAurA (cleaved from mCherry-xAurA) was incubated from purified xBuGZ (cleaved from YFP-xBuGZ) and MBP to assay AurA kinase activity in vitro. CBB, Coomassie Brilliant blue. (B) XEE was mock depleted or depleted (dep) with BuGZ antibody, and the BuGZ-depleted XEE was supplemented with the indicated purified proteins followed by xAurA immunoprecipitation and Western blotting analyses. r IgG, rabbit polyclonal IgG.