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Pirfenidone (PFD) suppresses β-catenin expression in HepG2 cells. HepG2 cells were treated with phosphate-buffered saline (control), an equivalent amount of DMSO, or 600 μmol/L PFD in DMSO for 48 h. (A) Treated HepG2 cells were harvested for Western blot analysis using β-catenin and p-β-catenin antibodies. (B) The Western blot densities were normalized to the density of GAPDH in each lane. (*** P<0.001 vs. control and DMSO).
