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. 2017 Nov 1;31(21):2162–2174. doi: 10.1101/gad.306324.117

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© 2017 Joo et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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Figure 4. — Taf1 promotes activator-independent multiround transcription. In vitro transcription was performed with TAF1 (YF157/YSW87; black) or taf1 (YF158/YSW90; gray) yeast nuclear extracts. NTPs were added for 3 min in single-round reactions (A) or 45 min for multiround transcription (B) in the presence or absence of transcription activator Gal4-vp16, as indicated. Transcripts were quantified, normalized, and plotted as the average value from three independent reactions. (C) The ratio of multiround transcripts in taf1 versus TAF1 extracts was divided by the same ratio in single-round reactions (see also Supplemental Fig. S5). Error bars show SD. (**) P = 0.014; (***) P = 0.006; (unmarked pairs) P > 0.01.