Figure 2.

WNK1 participates in mRNA export. (A) Fluorescent oligo-dT FISH for the detection of mRNA levels in control and WNK1-depleted cells. Alexa fluor 488-dT₍₂₃₎ was used as a probe, and DAPI was used for DNA staining. (B) Quantitation of the percentage of nuclear-localized mRNA over total. The integrated intensity of the nuclear fluorescent signal and the total fluorescent signal were calculated for every cell (n = 30; data are from two biological repeats), and the ratio between the two is presented graphically. Error bars represent SEM. (C) Heat map representing the log₂ ratio of nuclear over cytoplasmic (Nuc/Cyt) mRNA in control and WNK1-depleted cells for the top 20% of expressed genes. The darker shade indicates an increased Nuc/Cyt ratio. (D) Quantification of the fold change of Nuc/Cyt mRNA levels between siLUC and siWNK1 cells in the top 20% of expressed genes. Most genes in this set show an increase of Nuc/Cyt ratio upon WNK1 depletion.