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. Author manuscript; available in PMC: 2018 Jan 2.
Published in final edited form as: Bone. 2015 Nov 10;83:162–170. doi: 10.1016/j.bone.2015.11.007

Table 1.

Primers used for Real time RT-PCR analysis.

Gene Species GenBank accession no. Primer sequence PCR product length (bp)
S29 Human BC032813 5′-T CTCGCT CTT GTCGT GT CT GTTC-3′(S)
5′-ACACTGGCGGCACATATTGAGG-3′(AS)
75
CCN1 Human AF003114 5′-GCCAATGAAGCAGCGTTTCCC-3′(S) 5′-CAAT
GAGT CCCAT CACCCACACC-3′(AS)
171
CCN2 Human BT019794 5′-T GCGAGGAGT GGGTGTGTGAC-3′(S)
5′-TGGACCAGGCAGTTGGCTCTAATC-3′(AS)
125
CCN3 Human BC015028 5′-GAGCAGTGCCAATCTACAGCGAAG-3′(S)
5′-AGATGGAGAAGCAGGAAGGTCAGG-3′(AS)
138
CCN4 Human AB034725 5′-ACACTCATTAAGGCAGGGAAGAAG-3′(S)
5′-TCAGGACACTGGAAGGACACG-3′(AS)
185
CCN5 Human BC058074 5′-CACACCGAAGACCCACCTCCTG-3′(S)
5′-GCAGCAGCCACAGCCATCC-3′(AS)
151
CCN6 Human AY358350 5′-GGGTACAGGGCACTGGACCATTAG-3′(S)
5′-ACAGCATCCACAGCCATCTCTCAC-3′(AS)
167
COL2A1 Human BT007205 5′-TGGAGCAGCAAGAGCAAGGAGAAG-3′(S)
5′-CCGTGGACAGCAGGCGTAGG-3′(AS)
136
S29 Mouse NM_009093 5′-GGAGTCACCCACGGAAGTTCGG-3′(S)
5′-GGAAGCACTGGCGGCACATG-3′(AS)
108
Sox-9 Mouse NM_011448 5′-TCAGATGCAGTGAGGAGCAC-3′(S)
5′-CCAGCCACAGCAGTGAGTAA-3′(AS)
208
Acan Mouse L07049 5′-CCCGGTACCCTACAGAGACA-3′(S)
5′-ACAGTGACCCTGGAACTTGG-3′(AS)
215
Col2a1 Mouse BC082331 5′-GGGCAACAGCAGGTTCACATACAC-3′(S)
5′-TCAATAATGGGAAGGCGGGAGGTC-3′(AS)
120
Col10a1 Mouse NM_009925 5′-TTCTGCTGCTAATGTTCTTGA-3′(S)
5′-AATGCCTTGTTCTCCTCTTAC-3′(AS)
164
Bgn Mouse L20276 5′-ACCTGTCCCCTTCCATCTCT-3′(S)
5′-CCGTGTGTGTGTGTGTGTGT-3′(AS)
201
Dcn Mouse X53929 5′-CCAACATAACTGCGATCCCT-3′(S)
5′-TGTCCAAGTGGAGTTCCCTC-3′(AS)
214

S, sense; AS, antisense.