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. Author manuscript; available in PMC: 2018 Mar 1.
Published in final edited form as: Nat Med. 2017 Jul 31;23(9):1095–1101. doi: 10.1038/nm.4377

Figure 1. Human genetic variation significantly impacts the efficacy of RNA-guided endonucleases.

Figure 1

a, Schematic illustrating the genomic target (consisting of spacer and adjacent PAM element), RNA guide, and target variation. b, Fraction of residues for individual nucleotides containing variation in the ExAC dataset. c, Fraction of 2-nt PAM motifs altered by variants in the ExAC dataset. d, Percent of target variants at different allele frequencies for each CRISPR endonuclease. e, Cumulative percent of targets containing variants for each CRISPR endonuclease. f, Fraction of targets containing homozygous variants at different allele frequencies. The Mean and SEM for 5 CRISPR endonucleases is shown.