Table 1. Fraction of targets containing PAM-altering variants for five Cas enzymes.
n specifies the number of protein coding targets in the human exome for each enzyme. Orientation refers to the location of the PAM relative to the RNA guide complementary region of the target, and allele frequencies refer to the percentage of the ExAC population containing a particular PAM altering variant for a given target in the human exome.
| Whole-exome PAM variation by allele frequency (%) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Protein | PAM | Orientation | ≥10% | ≥1 | ≥0.1 | ≥0.01 | ≥0.001 | Total | n |
| AsCpf1-WT | TTTN | Left | 0.15 | 0.26 | 0.61 | 1.81 | 8.91 | 21.04 | 2702056 |
| SpCas9-VQR | NGA | Right | 0.11 | 0.25 | 0.69 | 2.28 | 11.39 | 23.19 | 9838603 |
| SpCas9-WT | NGG | Right | 0.16 | 0.37 | 1.13 | 3.82 | 17.46 | 32.61 | 10286445 |
| SaCas9-WT | NNGRRT | Right | 0.23 | 0.44 | 1.16 | 3.68 | 17.29 | 34.68 | 1938911 |
| SpCas9-VRER | NGCG | Right | 0.77 | 1.86 | 5.79 | 20.72 | 66.67 | 80.16 | 981524 |