Figure 4.

Both contralateral and ocular hypertensive eyes of Brown Norway rats were removed on day 7 and 42, post injury. Contralateral eyes were used as the control. Cryosections were immunostained by anti–phospho-Akt antibodies. We did not see any positive staining when primary antibodies were omitted (not shown). Data are a representation of at least four independent experiments. Comparable staining for phospho-Akt was seen in at least eight animals. NFL, nerve fiber layer; RGCL, retinal ganglion cells layer; IPL, inner plexiform layer.