Fig. 1.

Effect of drugs on CYP27A1 activity in the in vitro screening assay. Assay conditions are described in Materials and Methods. Control incubations contained only drug vehicle (1%, v/v). The results represent the mean ± S.D. of the three independent measurements. Drugs inhibiting CYP27A1 by ≥75% (the horizontal dashed line) are in bold. ND, not detectable; the limit of detection is 1% of cholesterol 27-hydroxylation. Superscripts indicate drug vehicles: D, dimethyl sulfoxide; M, methanol; W, water.