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. 2017 Dec 11;128(1):500–516. doi: 10.1172/JCI92742

Figure 4. Depletion of Mcl-1 downregulates HR and upregulates NHEJ.

Figure 4

(A) Schematic diagram of HR and NHEJ reporter systems. HR reporter is composed of 2 defective GFP genes that can be rescued only by HR, resulting in GFP fluorescence. In the NHEJ reporter, GFP is interrupted by an adenoviral exon (Ad2) and can be restored upon HindIII digestion and NHEJ repair. (B) HR activity was compared in WT and Mcl1–/– MEFs. Data represent the mean ± SD, n = 3 per group. **P < 0.01, by 2-tailed t test. (C) Mcl-1 was knocked down using Mcl-1 siRNA from U2OS DR-GFP cells, followed by analysis of HR activity. Data represent the mean ± SD, n = 3 per group. **P < 0.01, by 2-tailed t test. (D) NHEJ activity was compared in WT and Mcl1–/– MEFs. Data represent the mean ± SD, n = 3 per group. *P < 0.05, by 2-tailed t test.