Skip to main content
. 2017 Nov 27;128(1):323–340. doi: 10.1172/JCI93815

Figure 5. ADHFE1 induces epithelial-to-mesenchymal transition (EMT) and enhances invasion.

Figure 5

(A) Ingenuity pathway analysis showing pathways that are enriched for differently expressed genes comparing MCF12A cells with the ADHFE1 transgene versus vector control cells. Pathways are ranked by P values representing significance of enrichment. (B) Downregulation of E-cadherin and increased expression of mesenchymal markers in MCF10A and MCF12A cells overexpressing ADHFE1. (C) Cells with upregulated ADHFE1 develop a mesenchymal phenotype as shown by loss of cell-cell adhesion and a spindle-shaped morphology. Original magnification, ×200. Graphs below show quantitative analysis of this cell morphology. Shown is the mean ± SD for 10 counted areas (per 100 cells). (D) Increased invasion of cells overexpressing ADHFE1. Invasion was examined using the xCelligence system. Shown is the mean ± SD for triplicate experiments. *P < 0.05, compared with control cells (2-sided t test). Part of Figure 4B is shown in Figure 1D.