Skip to main content
. 2017 Nov 27;128(1):323–340. doi: 10.1172/JCI93815

Figure 9. ADHFE1 and cell-permeant D-2-hydroxyglutarate induce sphere formation of MCF10A and MCF12A cells.

Figure 9

(A) Upregulated ADHFE1 induces sphere formation and inhibits differentiation into terminal ducts in MCF12A cells. Control shows terminal ducts. Original magnification, ×40. (B) Cell-permeant 2-hydroxyglutarate (octyl-2HG) induces sphere formation and inhibits differentiation into terminal ducts. Original magnification, ×40. In A and B, data are the mean ± SD for triplicate experiments. *P < 0.05 versus control cells (2-sided t test). Cells were treated with 1 mM octyl-2HG or 1 mM PAMO (control). In A, terminal duct numbers for MCF10A cells were too low at baseline to be scored.