(A) Representative electromyogram recordings of responses to colorectal distension in rats fed RC, HFM, or HFM with rifaximin (HFM+Rif). (B) Mean amplitudes of abdominal muscle contractions are expressed as area under the curve (AUC) after baseline subtraction (n = 6 per group). *P < 0.05 compared with RC rats; #P < 0.05 compared with HFM. AUC, area under the curve; EMG, electromyographic activity; HFM, high-FODMAP diet; RC, regular chow. P < 0.05, by 2-tailed Student’s t test or 2-way repeated-measures ANOVA.