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. 2017 Dec 4;128(1):415–426. doi: 10.1172/JCI95837

Figure 5. Impaired development of γδT17 cells in PI3K-deficient mice.

Figure 5

(A) Flow cytometric profiles for CD3ε and TCRδ in total thymocytes from 0-day-old WT and Pik3cd–/–Pik3cg–/– mice. The total number of thymocytes is shown above each flow cytometric plot. Graph indicates the total number of γδT cells per mouse (n = 4–6). (B) Flow cytometric analysis of CD5 expression in thymic γδT cells (n = 4–6). (C and D) TCR-induced ERK (C) and Akt (D) phosphorylation in thymic Vγ4+ γδT cells from 1-day-old WT and Pik3cd–/– Pik3cg–/– mice. Graphs indicate the MFI relative to the nonstimulated control. (E) Intracellular staining for IL-17A and IFN-γ production in neonatal thymic γδT cells from 0-day-old WT and Pik3cd–/– Pik3cg–/– mice after stimulation with PMA and ionomycin. The number of IL-17A+ and IFN-γ+ γδT cells per mouse is shown (n = 3–6). (F) Number of Vγ4+ and Vγ6+ γδT cells (per mouse) in the indicated mice (n = 4–6). All data represent the mean ± SEM. *P < 0.05 and **P < 0.01, by 2-way ANOVA (C and D) and unpaired t test (A, E, and F). Data represent a single experiment using more than 7 neonatal mice per group.