Figure 7. ManNAc treatment protects mice from obesity-induced glucose intolerance and insulin resistance.

(A) Male WT mice were fed a HFD and either regular drinking water (control) or ManNAc-supplemented drinking water for 6 weeks. Plasma IgG was isolated, and its sialylation was evaluated by SNA-lectin blotting. Graph depicts the relative sialylation. n = 5. (B) BW and (C) fasting plasma glucose levels were measured, and (D) a GTT was performed. (E) Mice were continued on the HFD, and an ITT was performed 1 week later. n = 9. (A–E) Values represent the mean ± SEM. (A–C) *P < 0.05 and ****P < 0.001, by Student’s t test; (D and E) *P < 0.05, ManNAc versus control, by 2-way ANOVA with Tukey’s post-hoc test.